Trying to set up a domain to be hosted on a subdirectory on IIS Server , comes up with the following error:

This was due to the root domain using a different Application Pool then the subsite.

Use Advanced Settings of the parent site to change to the same application pool of the sub folder

HTTP Error 403.18 – Forbidden – The specified request cannot be processed in the application pool that is configured for this resource on the Web server.

Detailed Error InformationModule
IIS Web Core

Notification
BeginRequest

Handler
ExtensionlessUrlHandler-Integrated-4.0

Error Code
0x00000000

Requested URL
http://X.X.X.X:80/test/

Physical Path
D:\test\test\

Logon Method
Not yet determined

Logon User
Not yet determined

Most likely causes: •An ISAPI filter or custom module changed the URL to run in a different application pool than the original URL.
•An ISAPI extension (or custom module) used ExecuteURL (or ExecuteRequest) to run in a different application pool than the original URL.
•You have a custom error page that is located in one application pool but is referenced by a Web site in another application pool. When the URL is processed, it is determined by IIS that that it should have been processed in the first application pool, not the other pool.
•The Web site has multiple applications configured. The application this request is configured to run in is set to run in an application pool that does not exist.